#2749 QUANTIFICATION OF URINE PROTEINS FOR DISCOVERY OF BIOMARKERS FOR KIDNEY INJURIES BY DIA-PROTEOMICS

Abstract Background and Aims Recent advances in proteomics have enabled to quantify proteins comprehensively. We aimed establish a platform of urine quantitative for selection the biomarkers assessment health conditions. Since plasma varying amounts were present especially from proteinuric patients, we subtracted protein conteents total proteomes after discovery kidney injuries. On other hand, excreted may be indicators pathophysiological conditions body, suggesting that proteome profiles figure out early any diseases future. Method More than 120,000 samples (∼10 ml each) been collected ∼15,000 patients with various healthy volunteers (HV) stored -20C freezers. In this study, selected HV, diabetic (DM) or without microalbuminuria CKD patients. Proteins precipitated by methanol/chloroform precipitation method 1 urine. The fractions digested trypsin tryptic peptides purified C18 column chromatography. 200 ng each analyzed LC-MS (tims-TOFpro, Bruker) Data-independent acquisition (DIA) quantified DIA-nn tool. Major top 30 removed data kidney- urinary tract organ-derived normalized compare their proportions Results Approximately 2,500 DIA analysis. small, however, DM contents increased, resulting relative decreases derived organs. By subtraction proteins, comparable select candidates Comparison between HV elucidated differences, which might indicate changes Conclusion recent comprehensively, facilitating injuries also evaluation body.